Antimicrobial, and Antibiofilm Activities of Silver Doped Hydroxyapatite: A Novel Bioceramic Material for Dental Filling

Abstract Even though hydroxyapatite (HA) is the most common biocompatible material; it has limited antibacterial resistance. HA experiences a tailor-made structure depending on desired applications. In this regard, silver (Ag) of particular interest to inhibit wide spectrum pathogenic bacteria and other microorganisms. Silver doped (Ag-HA) was developed via wet co-precipitation with subsequent hydrothermal processing hinder growth multiplication microbes. Ag-HA demonstrated mono-dispersed nano-rods 70 nm length 7 diameters. dopant induced stresses within crystal lattice; maintained crystallographic no change. nanocomposite Ca/P value 1.238 compared 1.402 for virgin XPS spectroscopy. The reduction correlated partial replacement Ca +2 Ag +1 ; content reported be 1 atomic %. Elemental mapping using EDAX confirmed uniform dispersion ion lattice. Antimicrobial results indicated that, potent zone inhibition (ZOI) against Staphylococcus aureus , Candida albicans . Antibiofilm that at 10.0 µg/mL, experienced highest percentage S. C. 96.09%, 77.77%, respectively. an excellent disinfectant agent once had excited by UV light. curve assay, OD 600 Ag- were lower, showing repression impact It observed quantity cellular protein discharged from directly proportional concentration Ag-HA, which proves characteristics nanocomposite, explains creation holes in bacterial membrane producing oozing out proteins cytoplasm. achieved complete lysis cell malformation, decreasing total viable number, so outstanding antimicrobial encouraged tooth filling biomedical Graphical